The Olink® technology is an advanced platform designed to perform high-throughput and highly specific proteomic analysis with exceptional precision. Using Proximity Extension Assay (PEA) technology, Olink enables multiplexed protein quantification while maintaining high specificity and reproducibility. This cutting-edge system is widely used in biomedical research, biomarker discovery, and clinical applications.
Key features of Olink
Ultra-Specific & High-Throughput – Olink uses DNA-coupled antibodies that bind to target proteins in pairs, ensuring high specificity and reducing cross-reactivity. This enables the simultaneous analysis of up to 3,000 proteins in a single run.
Multiplexing Capability – Unlike traditional immunoassays, Olink allows for large-scale protein analysis while requiring only small sample volumes, making it ideal for studies involving limited or precious samples.
Quantitative & Reproducible – The system provides relative protein quantification with high precision, making it a powerful tool for longitudinal studies and biomarker validation.
Minimal Sample Requirement – Olink assays require as little as 1 µL of plasma, serum, or other biofluids, making it suitable for clinical and translational research
Assay methodology
- Antibody Binding: Two oligonucleotide-labeled antibodies bind to the same target protein in close proximity.
- Proximity Extension & DNA Polymerization: If both antibodies are bound, their DNA tags hybridize and are extended by DNA polymerase, creating a unique sequence.
- qPCR or NGS-Based Detection: The DNA sequence is amplified and quantified using real-time PCR (qPCR).
- Data Analysis: Results are processed using Olink Insight Software, offering detailed proteomic insights that can be integrated into statistical and bioinformatics tools.
By offering exceptional sensitivity, specificity, and scalability, Olink technology is revolutionizing proteomics and precision medicine, helping researchers unlock new biological insights and develop more effective therapeutic strategies.